Part:BBa_K360041:Experience

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BBa_K238013 ETH Zürich iGEM 2012

ETH Zürich iGEM 2012 We used the part BBa_K238013, on which this part is based on, as a blue light inducible repression system. Fortunately the research team of Natalie Tschowri at FU Berlin [http://www.ncbi.nlm.nih.gov/pubmed/22783906 recently published] new results including a DNA footprint for the inverted repeats in the YcgZ promoter. We compared the inverted repeats in their research to the ones indicated on BBa_K238013, and therefore also on BBa_K360041, and we found them to be different. A) The DNA footprint done in the research of [http://www.ncbi.nlm.nih.gov/pubmed/22783906 Natalia Tschowri 2012] that shows the binding regions / inverted repeats for BluR (YcgE). B) Shift Assay: Point mutations in the determined binding regions drastically decrease DNA binding of BluR (YcgE).

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